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TRAPase positive multinucleated osteoclasts (≥3 nuclei) attached to the cancellous bone region (350–1750 μm from the distal growth plate) were quantified
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The primary pathways for clearing L-OOHs in ferroptosis are the cystine/glutamate antiporter system-glutathione-glutathione peroxidase 4 axis (System Xc-/GSH/GPX4 axis), the ferroptosis suppressor protein 1-coenzyme Q10-nicotinamide adenine dinucleotide phosphate axis (FSP1/CoQ10/NADPH axis), and the GTP cyclohydrolase 1-tetrahydrobiopterin-dihydrofolate reductase axis (GCH1/BH4/DHFR axis) (Figure 1C)
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