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To confirm that the pem1 Δ / Δ pem2 Δ / Δ mutant’s lack of growth in choline-free media correlates with loss of PC synthesis, total lipids were extracted from cells growing in minimal medium lacking a source of choline, and analyzed by thin layer chromatography (TLC) (Supplementary Figure S1)
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● Regulate appetite and metabolism: By supporting the gut-brain axis and metabolic pathways, BPC-157 may help regulate appetite and improve overall metabolic function—key factors in achieving and maintaining a healthy weight
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