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The inhibition of 3.7 nM TGR with 250 µM inhibitor, 100 µM NADPH, and a 15 min pre-incubation at RT was measured to compare to the inhibition after the jump dilution
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Interestingly, although PCTX does not cause direct mitochondrial DNA damage, mitochondria have been suggested as potential mediators for PCTX toxicity, as swollen and vacuolated mitochondria have been observed in myelinated and unmyelinated sensory nerves, thus the authors conclude that alterations in mitochondria contribute to PCTX-induced pain instead of dysfunctions of axonal microtubules (Flatters and Bennett 2006
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