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In a double-blind, randomized, controlled trial involving 101 patients who underwent major hepatectomy, the perioperative administration of pentoxifylline significantly increased the regenerative capacity of small remnant livers (remnant liver to body weight ≤ 1.2%)

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Cell pellets were resuspended in 0.6 ml cold water/methanol (1:2) and subjected to three freeze–thaw cycles before sonication in a wet ice bath for 15 min (cycles: 1 min pulse followed by 1 min pause)