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A recent review highlighted concerns with IV use, including rare anaphylaxis and potential hepatotoxicity (PMC narrative review)

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TCEP Tris(2-carboxyethyl)phosphine the stock solution of the internal standard (IS, 13 C 2 , 5 N-GSH-d5-NEM) was prepared by reacting 2 mmol/L glutathione-(glycine- 13 C 2 , 15 N) (Cambridge Isotope laboratories, MA, USA) with 50 mmol/L d5-NEM in 50 mmol/L AA, with subsequent extraction of residual d5-NEM with dichloromethane