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The cell lysates were incubated for 20–25 min on ice followed by centrifugation at 14,000 rpm for 15 min at 4 °C
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enrichment of K29-, K48-, or K63-linked ubiquitin chains and their conjugated proteins was performed using GST-Trabid-NZF1 (UBPBio, J4470), FLAG K48-TUBE HF (LifeSensors, #UM607), or FLAG K63-TUBE (LifeSensors, #UM604), respectively
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