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Peripheral blood mononuclear cells were isolated by Ficoll® density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 × 10 6 cells/mL for 2 h in serum-free media at 37 °C [20]

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20 Examples include a monochlorinated BODIPY-based ratiometric fluorescent sensor developed by Niu and co-workers, 21 a bis -spiropyran ligand developed for cellular imaging, 22 a quantum-dot-based off-on fluorescent probe 23 and cyanine-based fluorescent probes reported by Yin et al