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Interestingly and not part of our initial design, which focused on direct reactivity with ROS, OH Py 2 N 2 showed the potential to activate cellular antioxidant defense capacity via activation of the Nrf2 (Nuclear factor erythroid 2-related factor 2) signaling pathway in neuronal cell culture along with low toxicity, high metabolic stability, and proving to be highly water soluble (Johnston et al., 2019)

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Taken together, these observations constitute highly consistent evidence supporting the notion that free radical-associated effects of Aβ42 require Met in position 35 of this neurotoxic peptide, and if Tyr10 is involved, we suggest that its role is likely relatively limited