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The negative controls are shown in bottom middle and right hand panels - for negative control 1 purified ab125066 was used at a dilution of 1/500 followed by an Alexa Fluor ® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500
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Together, these data indicate that PIF4’s DNA-binding activity makes only a minor contribution to thermomorphogenesis
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