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intraperitoneally) using the sterile technique, shaving the hair of the left pelvic and proximal thigh region, the rats were placed in the right lateral position (lateral decubitus position), and a longitudinal incision of 2 cm was made from the anterosuperior iliac spine to the level of the transition of the upper to the middle third of the thigh

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Application Protocol for Research Use Standard application of a GHK-Cu topical serum in research settings follows a straightforward five-step protocol: Cleanse — wash the target area with a gentle cleanser and pat dry Apply — dispense a thin layer of serum onto the skin Absorb — allow 2–3 minutes for full absorption before applying moisturizer or sunscreen Frequency — apply once or twice daily for consistent research protocols Duration — the published clinical studies used 12-week protocols to demonstrate measurable outcomes Researchers investigating combination approaches sometimes layer topical GHK-Cu with other cosmetic actives or research peptides