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The 15N-1H CSP-guided docking in principle can generate reasonable starting model 121 , demonstrated in protein-protein or protein-RNA systems 108,109,110,111 , that can further be subjected to long-timescale all-atom MD simulations, allowing to probe the complex’s stability and interaction mechanism in atomic detail
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Briefly, bone marrow was flushed from the femurs and tibias of 8–10-week-old C57BL/6J, Gclm +/+ and Gclm −/− mice using PBS and a 26G needle
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