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Innovative Health Solutions (now NeurAxis) obtained FDA De Novo clearance for two paVNS devices: the NSS‐2 Bridge for reducing symptoms of opioid withdrawal in patients with substance use disorder (FDA ref.: DEN170018) in 2017, and the IB‐Stim for functional abdominal pain associated with IBS (FDA ref.: DEN180057) in 2018
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After incubation for 2 h on ice, the protein solutions were exchanged into buffer (50 mM HEPES 7.3, 150 mM KCl) using a PD-10 column (Cytiva, 17085101) following the manufacturer’s instructions
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