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The FDA also notes that 28 days is the assumed opened-vial period for this kind of container unless the label specifies something else
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After removal of the supernatant, the aggregates were resuspended in 3.5 ml/million cells of differentiation medium comprised of RPMI 1640 (Life Technologies) with B-27 either with or without insulin (RPMI/B-27 +/-) (Life Technologies) depending on the cell line and 3 or 4 μM CHIR99021 (BOC Sciences)
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