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Layering – Apply it after cleansing and toning the skin to allow faster absorption

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After deep anaesthesia with isoflurane (Attane Vet, 1,000 mg g −1 , ScanVet, Piramal Critical Care) mice were decapitated and the brain was removed and submerged in ice-cold aCSF (124 mM NaCl, 5 mM KCl, 1.2 mM Na 2 HPO 4 ·2H 2 O, 2.7 mM CaCl 2 ·2H 2 O, 1.2 mM MgSO 4 (anhydrous)), 10 mM dextrose, 26 mM NaHCO 3 was adjusted to pH 7.4 and an osmolarity of 298–302 mOsm kg −1 after saturation with carbogen (95% O 2 /5% CO 2 )