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Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts
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Treatment of certain inherited disorders: Some inherited metabolic disorders, such as methylmalonic acidemia and homocystinuria, may benefit from hydroxocobalamin treatment due to its role in converting methylmalonyl-CoA to succinyl-CoA and facilitating the conversion of homocysteine to methionine
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