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The culture was lysed in 20ml of binding/wash buffer and loaded onto a column containing 1ml of HisLink™ Resin
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For commensals such as E coli , the tnaA gene that is annotated as a tryptophanase and reported to break down tryptophan to indole and pyruvate [[60], [61], [62]], has also been reported to be important for catalyzing the metabolism of cysteine to H 2 S [63]
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