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Polyethylenimine (PEI) NPs delivering GLP-1 genes have improved β-cell function and endogenous insulin secretion, and combined nanoparticle systems delivering insulin and GLP-1 agonist genes exhibited synergistic improvements in glycaemic control [219, 220]
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In fact, many GST pull-downs use cell lysates to try to discover new interactions with their protein of interest (Figure 1)
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