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Techniques such as high performance liquid chromatography are used to verify peptide identity and confirm high purity levels
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Figure 9 illustrates this point: assuming two different protein–protein complexes, A–B and X–Y with similar energies of their bound state but different energies of their free states, any model considering only the bound state will predict similar binding affinities for those two complexes while, experimentally, they will have different affinities owing to the differences in their respective free states, The free state contribution is typically neglected in docking
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