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Intriguingly, when System Xc- is inhibited, cells respond with a compensatory transcriptional upregulation of SLC7A11 [10]
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An equivalent amount of protein from each animal was mixed with 4 × sample buffer with or without 2-mercaptoethanol, then separated by SDS–polyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)
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