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A notable example is the development of chemically defined, animal serum-free 3D primary human hepatocyte spheroid cultures, which eliminate fetal bovine serum, a variable, poorly characterized animal product, and demonstrate comparable viability and cytochrome P450 function, thus improving reproducibility and ethical compliance in long-term studies ( Finally, an important future direction for our work is the inclusion of serum samples from diverse ethnic and vulnerable populations, as large registry and cohort studies have shown that DILI severity can differ across populations, presumably because of individual-specific predispositions, environmental factors, cultural lifestyle, and drug-use patterns

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5-Amino-1MQ Dosage in Published Research Doses reported in the published preclinical literature are expressed in mg/kg/day administered by oral gavage in mouse models, with cell-based studies reporting micromolar concentrations in culture medium