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In separate assays, serum-starved PC3 cells were transfected with AR-Flag variant 2 and pmCherry or pmCherry-RanQ69L and treated with Sema4D-Fc (2 μg/ml, Bio-techne), DHT (Sigma) or vehicle control for 1.5 h or 1 h respectively
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Liquid chromatography-based assays The most commonly used HPLC technique is based on fluorescent labelling
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