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When the oxidized enzyme is mixed with equimolar NADH plus cyt b 5 in the electron-spin-resonance-equipped stopped-flow apparatus, NAD + -FAD •− is observed: cyt b 5 reductase (FAD ox ) + cyt b 5 3+ + NADH → cyt b 5 reductase (NAD + -FAD •− ) + cyt b 5 2+ + H + [63]
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