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Slow-acting biogenic amine neurotransmitters are produced by sequential modification of aromatic amino acids: from tyrosine, tyrosine hydroxylase (TH, the rate-limiting enzyme using Fe²⁺ and tetrahydrobiopterin) produces L-DOPA, which DOPA decarboxylase converts to dopamine, dopamine β-hydroxylase to norepinephrine, and phenylethanolamine N-methyltransferase (PNMT) to epinephrine
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