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berghei infected blood was harvested from mice with 5–15% parasitemia, diluted in complete medium [RPMI 1640 (Gibco ), 25% heat inactivated fetal bovine serum (Gibco ), 50 IU/ml of neomycin (Sigma)] and cultured for 24–26 h at 37°C in an atmosphere of 10% O 2 , 5% CO 2 and 85% N 2 gas mixture
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